A New Survey Method for Citrus Tristeza Virus Disease Assessment

Survey methods for assessment of citrus tristeza virus incidence.

ABSTRACT The assessment of citrus tristeza virus incidence by sampling involves laboratory testing by enzyme-linked immunosorbent assay of leaf material collected in the field. Using field data and computer simulation, methods of field data collection were compared. One method was similar to that used by the Central California Tristeza Eradication Agency, in which 4 to 6% of the trees in a plan...

Citrus tristeza virus-host interactions

Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes sev...

The Complex Genetics of Citrus tristeza virus

The 2000 x 11 nm long bipolar flexuous filamentous particles of Citrus tristeza virus (CTV) (genus Closterovirus, family Closteroviridae) (Figure 1) contain a single-stranded positive-sense RNA genome of 19.3 kb, which is encapsidated in two different capsid proteins that coat the opposite ends of the virions [1, 2]. CTV is the largest identified RNA virus infecting plants and the second larges...

Past and future of a century old Citrus tristeza virus collection: a California citrus germplasm tale

Citrus tristeza virus (CTV) isolates collected from citrus germplasm, dooryard and field trees in California from 1914 have been maintained in planta under quarantine in the Citrus Clonal Protection Program (CCPP), Riverside, California. This collection, therefore, represents populations of CTV isolates obtained over time and space in California. To determine CTV genetic diversity in this conte...

Molecular Virology and Pathogenicity of Citrus tristeza virus