A new single run polymerase chain reaction assay for cyclosporiasis in immunocompromised patients

نویسندگان

چکیده

causes human intestinal cyclosporiasis. It is more common in the immunocompromised patients and mainly seen people living with HIV/AIDS (PLHA), post-renal transplant (PRT) children (IC). Diagnostic microscopy for oocysts of parasite less sensitive, requiring examination multiple stool samples. Here we developed a new single run polymerase chain reaction (PCR) assay detection it was used to know hospital based prevalence A cross-sectional study conducted from June 2016 October 2020 tertiary care teaching hospital. amplification PCR-based diagnostic . Stool samples were collected 121 PLHA, 135 PRT 79 (IC) other than PLHA PRT. All examined presence as well tested PCR assay. Modified Ziehl-Neelsen staining concentrated smear did not reveal species any specimen. However, detected 2 specimens – one patient another patient, giving 0.6% (2/335), 0.8% (1/121) 0.7% (1/135) IC. Cyclosporiasis infrequent southern part India. The by us simple cost effective molecular

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ژورنال

عنوان ژورنال: IP international journal of medical microbiology and tropical diseases

سال: 2021

ISSN: ['2581-4753', '2581-4761']

DOI: https://doi.org/10.18231/j.ijmmtd.2021.042