A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening
نویسندگان
چکیده
منابع مشابه
A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods.
We have developed a simple two-dimensional YAC pooling strategy to facilitate YAC library screening via STS and Alu-PCR approaches. The method has been implemented using the human total genomic YAC library of Olson and coworkers, and its validity tested by isolation of many chromosomes 19- and 21-specific YACs. The Alu-PCR approach is notable in that it is hybridization-based, such that PCR pri...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2010
ISSN: 1471-2164
DOI: 10.1186/1471-2164-11-692