A New Fluorescence-Based Method Identifies Protein Phosphatases Regulating Lipid Droplet Metabolism

A New Fluorescence-Based Method Identifies Protein Phosphatases Regulating Lipid Droplet Metabolism

In virtually every cell, neutral lipids are stored in cytoplasmic structures called lipid droplets (LDs) and also referred to as lipid bodies or lipid particles. We developed a rapid high-throughput assay based on the recovery of quenched BODIPY-fluorescence that allows to quantify lipid droplets. The method was validated by monitoring lipid droplet turnover during growth of a yeast culture and...

A new role of p53 in regulating lipid metabolism.

Dear Editor, The p53 tumor suppressor maintains the normal cell growth and genomic stability by launching cell cycle arrest, DNA repair, or apoptosis in response to DNA damage or other forms of cellular stress. Recent studies also suggest that p53 is capable of much broader cellular functions, including the regulation of energy metabolism and autophagy. However, the role of p53 in regulating li...

Thematic review series: Lipid droplet synthesis and metabolism: from yeast to man. Lipid droplet-based storage fat metabolism in Drosophila.

The fruit fly Drosophila melanogaster is an emerging model system in lipid metabolism research. Lipid droplets are omnipresent and dynamically regulated organelles found in various cell types throughout the complex life cycle of this insect. The vital importance of lipid droplets as energy resources and storage compartments for lipoanabolic components has recently attracted research attention t...

Lipid Droplet-based Storage Fat Metabolism in Drosophila

Lipid Metabolism: Ceramide Transfer Protein Adds a New Dimension

Just as non-lipidologists were becoming reconciled to the complexities of networks of ceramide metabolism and the diverse roles of ceramide in cell regulation and membrane structure, a new study reveals a new layer of regulation of ceramide through the actions of a ceramide binding/transfer protein.