A New Apparatus for Preparative Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
A new apparatus for preparative gel electrophoresis.
paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi ca...
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Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
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‘T1HE INTRODUC”ION of starch gel as an electrophoresis medimn by Smithies k4) arkecl a significant advance in the analysis of serum proteins. The increased resolving power afforded by this medium is indicated by the fact that as many as 30 distinct components can be electrophoretically separated; only 5 or 6 are usually observed on paper or in free-solution electrophoresis. Acrylamide gel (2, 3...
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We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods. In this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrop...
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Preparative polyacrylamide gel electrophoresis has been used in the final stages of purification to prepare horse serum cholinesterase which was at least 9.5% pure, as judged by analytical polyacrylamide gel electrophoresis. The starting material, already purified about 109-fold by the Strelitz method, was purified an additional 89-fold by the procedure. The method involved Sephadex G-ZOO and S...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1972
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.36.2247