A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) multi-class method was developed and validated for the determination of 27 antibiotic residues from five classes (macrolides, sulfonamids, quinolones, lincosamids, diaminopyrimidines) in milk powder whey which are commonly used as raw material many products food industry. A simple, inexpensive fast sample preparation based on extraction samples with methanol is main advantage method. The validation performed following requirements outlined European Commission Decision 2002/657/EC, required procedure methods analysis official audits Europe Turkey. Matrix effect uncertainties related instrumental were minimised by using matrix-matched calibration. capable detecting at very low concentrations.