A Molecularly Imprinted Polymer-Disposable Pipette Tip Extraction-Capillary Electrophoresis (MISPE-DPX-CE) Method for the Preconcentration and Determination of Scopolamine in Synthetic Urine Samples

Alcoholic beverages contaminated with scopolamine (SCP) are often employed for criminal purposes due to their sedative effect. The determination of the residual levels SCP in body fluids (e.g., urine) can help track possible victims induced ingestions. Biological sample analysis usually requires a preconcentration step enhance detectability and provide clean-up. Molecularly imprinted polymers (MIPs) lieu conventional solid sorbents represent an enhancement selectivity, specific recognition sites. Additionally, adaptation solid-phase extraction (SPE) cartridge into disposable pipette tip (DPX) contributes miniaturization preparation step. Herein, analytical method synthetic urine samples via integration molecularly (MISPE) DPX as prior capillary electrophoresis (also known MISPE-DPX-CE) is presented. elution steps were optimized using factorial design. Using conditions, factor 20 was obtained, leading working range 0.5–6 µM LOD 0.04 repeatability 6.4% (n = 7) adequate recovery values (84 101%) proposed MISPE-DPX-CE approach successfully applied selective extraction, preconcentration, samples.