A modified immunoblot method to identify substrates of protein kinases
نویسندگان
چکیده
منابع مشابه
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The identification of all the substrates of every protein kinase is one of the major challenges of post-genomic research. Here we review a powerful method for tackling this problem that we have developed over the last 5 years. The method has so far been used to identify novel substrates for eight different protein kinases, demonstrating that it is of general utility. Importantly, the method can...
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Phosphorylation is a ubiquitous protein modification important for regulating nearly every aspect of cellular biology. Protein kinases are highly conserved and constitute one of the largest gene families. Identifying the substrates of a kinase is essential for understanding its cellular role, but doing so remains a difficult task. We have developed a high-throughput method to identify substrate...
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ژورنال
عنوان ژورنال: The Journal of Microbiology
سال: 2011
ISSN: 1225-8873,1976-3794
DOI: 10.1007/s12275-011-0465-y