A model for metalloprotein-catalysed ADP, ATP transport in mitochondria
نویسندگان
چکیده
منابع مشابه
Rickettsial permeability. An ADP-ATP transport system.
The obligate intracellular parasitic bacterium, Rickettsia prowazeki, has a carrier-mediated transport system for ADP and ATP. The transport of nucleotides was measured by membrane filtration assays; the assay was shown not to harm the relatively labile rickettsiae. The nucleotide transport system was shown to reside in the rickettsiae, not in the contaminating yolk sac mitochondria of the prep...
متن کاملTransport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex.
Members of the mitochondrial carrier family such as the ADP/ATP carrier (AAC) are composed of three structurally related modules. Here we show that each of the modules contains a mitochondrial import signal recognized by Tim10 and Tim12 in the intermembrane space. The first and the second module are translocated across the outer membrane independently of the membrane potential, DeltaDeltapsipsi...
متن کاملTransport of ADP/ATP carrier into mitochondria. Precursor imported in vitro acquires functional properties of the mature protein.
Precursor to ADP/ATP carrier synthesized in vitro is transferred into isolated mitochondria to a protease-resistant location. This process requires an electrical potential across the inner membrane. We show now that precursor imported in a cell-free system exhibits the same resistance to protease as the mature endogenous carrier. Furthermore, transferred protein, but not receptor-associated pre...
متن کاملEffect of creatine kinase activity on mitochondrial ADP/ATP transport. Evidence for a functional interaction.
Mitochondrial adenine nucleotide translocation was measured in the presence of creatine or creatine phosphate to test the proposed functional couple between the nucleotide translocase and creatine kinase. Rat heart mitochondria were preloaded with the corresponding nucleotide which reacts with creatine kinase only after it is transported across the mitochondrial membrane; namely, radioactive AT...
متن کاملRotational diffusion of the ADP/ATP translocator in the inner membrane of mitochondria and in proteoliposomes.
The ADP/ATP translocator was selectively labeled with the triplet probe eosin-5-maleimide (EMA) after pretreatment with N-ethylmaleimide in beef heart mitochondria, as reported previously for submitochondrial particles (Müller, M., Krebs, J. J. R., Cherry, R. J., and Kawato, S. (1982) J. Biol. Chem. 257, 1117-1120). The EMA binding was completely inhibited by carboxyatractylate. 0.7-1.1 molecul...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1983
ISSN: 0014-5793
DOI: 10.1016/0014-5793(83)80926-0