A microporous walled micro-capillary film module for cation-exchange protein chromatography

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Functionalized micro-capillary film for the rapid at-line analysis of IgG aggregates in a cell culture bioreactor

A micro-capillary film has been developed that offers the potential for an at-line analytical tool for rapid aggregate analysis during biopharmaceutical antibody production. A non-porous walled micro-capillary film (NMCF) with cation exchange functionality was demonstrated to act as a chromatography medium that could be operated with high linear fluid velocities and was highly resistant to bloc...

متن کامل

Capillary isoelectric focusing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants.

We have developed two assays for complete analysis of hemoglobins (Hbs) in the field of hemoglobinopathies: a high-performance cation-exchange liquid chromatography (HPLC) assay on the weak cation-exchanger Poly Cat A and a two-step capillary isoelectric focusing (CIEF) assay on the neutral-coated capillary from Beckman in a narrow pH gradient. The resolution was satisfactory for both HPLC and ...

متن کامل

Ion-exclusion/cation-exchange chromatography (IEC/CEC) on a weakly acidic cation-exchange resin using on acidic eluent,

Ion-exclusion/cation-exchange chromatography (IEC/CEC) on a weakly acidic cation-exchange resin using on acidic eluent, developed by Tanaka et al. in 1994, has been employed to monitor the quality of environmental water samples such as acid rainwater and river water, to analyze body fluid samples such as saliva, and to evaluate water purification by photocatalytic materials.1–8 One advantage of...

متن کامل

Single plane illumination module and micro-capillary approach for a wide-field microscope.

A module for light sheet or single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures, e.g., multi-cellular tumor spheroids (MCTS). The SPIM excitation module shapes and deflects the light such that the sample is illuminated by a light sheet perpendicular to the detection path of the micros...

متن کامل

Optimizing protein separations with cation exchange chromatography using Agilent Buffer Advisor

This Technical Overview shows that the Agilent Buffer Advisor software in combination with the Agilent 1260 Infi nity Bio-inert Quaternary LC System is an ideal solution for automated protein separation by ionic strength gradients. Usually, pH scouting using premixed two-component gradients is time-consuming and workintensive. Dynamically mixed four-component gradients calculated by the Buffer ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Membrane Science

سال: 2014

ISSN: 0376-7388

DOI: 10.1016/j.memsci.2014.04.049