A Microneedle-Microplate Platform to Detect Biomarkers in the Skin
نویسندگان
چکیده
This research aimed to integrate an established signalquantification technique with immunocapture microneedle arrays (MNAs) improvebiomarker-based disease diagnostics in the skin. In this miniaturized sandwichenzyme-linked immunosorbent assay (ELISA) platform, capture antibody wasimmobilised onto surface of microneedles which, when inserted superficiallyinto porcine skin, captured endogenous immunoglobulin G (IgG) intradermally. Signal development was achieved by incubating ino-phenylenediamine (OPD) solution a 384-well microplate and measuring theabsorbance spectrophotometer at 450 nm. allowsfor rapid biomarker detection high-throughput processing. Immunocapture microneedledevices such as these can be easily adapted for targeting different biomarkersor multiplexed leaving plenty scope future work optimisation. 
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ژورنال
عنوان ژورنال: British journal of pharmacy
سال: 2022
ISSN: ['2058-8356']
DOI: https://doi.org/10.5920/bjpharm.1164