A METHOD FOR PRODUCING SPECIFIC ANTISERA WITH SMALL DOSES OF IMMUNOGEN

Studies on the Production of Specific Hyperimmune Antisera Against Type A F.M.D. Virus

Antisera specific for D2 dopamine receptors.

Antisera have been raised against two peptides from the sequence of D2 dopamine receptors: peptide 1 from the predicted second extracellular loop and peptide 2 from the predicted third intracellular loop. The antisera recognize specifically a 95 kDa band in Western blots of several bovine brain regions, which corresponds to the denatured D2 dopamine receptor, whereas in recombinant CHO cells ex...

unveiling a specific immunogen protein in a. baumannii

objectives: acinetobacter baumannii is a gram-negative, non-motile aerobic bacterium known as a nosocomial pathogen resisting often to broad range of antibiotics. the pathogen is a serious agent of mortality and morbidity in hospital particularly among immunocompromise patients. treatment and control of its infections is complicated owing to its high antibiotic resistance, survival in various e...

Negative purification method for the selection of specific antibodies from polyclonal antisera.

We developed a protocol to remove non-specific antibodies from polyclonal antisera by adsorption on non-target antigens immobilized on nitrocellulose membranes. This "negative" purification method is simple and provides better immunoreagents than the blocking of nonspecific antibodies in solution or the enrichment of specific antibodies on nitrocellulose membranes. For routine applications, thi...

Segmental volvulus of small intestine: report of a case with an innovative method of surgery

  Absract   Intestinal malrotation is a general term used for any deviation from normal embryonic GI tract rotations. This abnormality, denotes a spectrum of conditions (non rotation, incomplete rotation) that according to the severity of anatomic consequence, can result in complications found in infancy, childhood, adolescence, adulthood or old age. This article, reports another case of intest...