A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA
نویسندگان
چکیده
منابع مشابه
Reference datasets of tufA and UPA markers to identify algae in metabarcoding surveys
The data presented here are related to the research article "Multi-marker metabarcoding of coral skeletons reveals a rich microbiome and diverse evolutionary origins of endolithic algae" (Marcelino and Verbruggen, 2016) [1]. Here we provide reference datasets of the elongation factor Tu (tufA) and the Universal Plastid Amplicon (UPA) markers in a format that is ready-to-use in the QIIME pipelin...
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The light microclimate of phototrophic endoliths growing within the scleractinian corals Porites cylindrica and Montipora monasteriata was described by scalar irradiance microprobe measurements within different layers of the coral skeleton. Characterisation of the pigments in individual layers was done by reflectance spectroscopy with fibre-optic radiance microprobes. The spectral measurements ...
متن کاملMulti-marker metabarcoding of coral skeletons reveals a rich microbiome and diverse evolutionary origins of endolithic algae
Bacteria, fungi and green algae are common inhabitants of coral skeletons. Their diversity is poorly characterized because they are difficult to identify with microscopy or environmental sequencing, as common metabarcoding markers have low phylogenetic resolution and miss a large portion of the biodiversity. We used a cost-effective protocol and a combination of markers (tufA, 16S rDNA, 18S rDN...
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ژورنال
عنوان ژورنال: BMC Ecology
سال: 2016
ISSN: 1472-6785
DOI: 10.1186/s12898-016-0068-x