A luciferase lysis assay reveals in vivo malignant cell sensitization by phosphoantigen prodrugs

Expression and Purification of the luciferase enzyme and in Vivo ATP Assay

Introduction: Gene expression and purification of luciferases from the firefly, Lampyris turkestanicus, and optimization of cellular ATP measurements were performed. Methods: cDNA encoding luciferases from Lampyris turkestanicus was transferred from pQE30 vector into pET28a expression vector and pLtu28 was built. Newly constructed vector was expressed in E. coli XL1 Blue and the recombinant l...

In vivo evidence for dendritic cell lysis by NK cells

By using an experimental model of anticancer vaccination, we have recently lent support to the assumption, so far only sustained by in vitro data, that natural killer cells can restrain less immunogenic, allegedly tolerogenic, dendritic cells (DCs). This in vivo selection of immunogenic DCs appears to depend on perforin and to be associated with a more protective tumor-specific T lymphocyte res...

LightSwitchTM Luciferase Assay System

expression and purification of the luciferase enzyme and in vivo atp assay

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...

Bioluminescence in soybean root nodules: Demonstration of a general approach to assay gene expression in vivo by using bacterial luciferase.

Two plasmid vectors pFIT001 and pPALE001, containing luxAB genes encoding bacterial luciferase [alkanal, reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] from Vibrio harveyi, have been constructed. Escherichia coli carrying derivatives of pFIT001 with DNA inserts in the unique EcoRI site located in luxB form "dark" colonies that can be readily distinguished from t...