منابع مشابه
A low-cost method for brain slice cultures.
Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphological...
متن کاملOrganotypic Brain Slice Cultures: a Review
In vitro cell cultures are an important tool for obtaining insights into cellular processes in an isolated system and a supplement to in vivo animal experiments. While primary dissociated cultures permit a single homogeneous cell population to be studied, there is a clear need to explore the function of brain cells in a three-dimensional system where the main architecture of the cells is preser...
متن کاملa low-cost method for optical tomography
introduction: in this study, arrangement of a low-cost optical tomography device compared to other methods such as frequency domain diffuse tomography or time domain diffuse tomography is reported. this low-cost diffuse optical imaging technique is based on the detection of light after propagation in tissue. these detected signals are applied to predict the location of in-homogeneities inside p...
متن کاملA simple method for multiday imaging of slice cultures.
The organotypic slice culture (Stoppini et al. A simple method for organotypic cultures of nervous tissue. 1991;37:173-182) has become the method of choice to answer a variety of questions in neuroscience. For many experiments, however, it would be beneficial to image or manipulate a slice culture repeatedly, for example, over the course of many days. We prepared organotypic slice cultures of t...
متن کاملNimodipine enhances neurite outgrowth in dopaminergic brain slice co-cultures.
Calcium ions (Ca(2+)) play important roles in neuroplasticity and the regeneration of nerves. Intracellular Ca(2+) concentrations are regulated by Ca(2+) channels, among them L-type voltage-gated Ca(2+) channels, which are inhibited by dihydropyridines like nimodipine. The purpose of this study was to investigate the effect of nimodipine on neurite growth during development and regeneration. As...
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ژورنال
عنوان ژورنال: Journal of Pharmacological Sciences
سال: 2007
ISSN: 1347-8613,1347-8648
DOI: 10.1254/jphs.sc0070119