A kinetic determination of ammonia in plasma
نویسندگان
چکیده
منابع مشابه
Two-point determination of plasma ammonia with the centrifugal analyzer.
A simple, convenient, and rapid method for determining ammonia in plasma by the glutamate dehydrogenase reaction is described for the centrifugal analyzer. The measuring principle is fixed-time, with NADH as the coenzyme. ADP is added to stabilize glutamate dehydrogenase and prevent interference from endogenous plasma ADP. The reaction is linear to 400 mumol of ammonia per liter. The plasma sam...
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A method is described for the colorimetric determination of ammonia in plasma by a batch ion-exchange procedure. The method combines separation of ammonium ion, using a strongly acidic cation-exchange resin, with a sensitive colorimetric procedure which simultaneously elutes and colorimetrically reacts with the ammonium ion to produce a stable color. Interference by basic amino acids is negligi...
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The glutamate dehydrogenase (GLDH, EC 1.4.1.3) reaction is almost universally used for the determination of ammonia. Some kit manufacturers specify either heparinized or EDTA plasma, whereas others recommend EDTA only, but without saying why. The procedure in Volume 9 of Selected Methods in Clinical Chemisty (1) requires EDTA but does not discuss the consequences of substituting heparin. We hav...
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The reduction capacity of ammonia while present even at ppm level can be demonstrated by the increased encounter probability between ammonia and methylene blue dye (MB+) incorporated in nanomordenite and Na-MOR zeolites. The rate of reduction methylene blue dye by ammonia on the surface nanomordenite zeolite is faster than Na-mordenite (Na-MOR) zeolite.Because nanomordenite zeolite with high si...
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 1974
ISSN: 0009-8981
DOI: 10.1016/0009-8981(74)90223-x