A guide to super-resolution fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
A guide to super-resolution fluorescence microscopy
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new ap...
متن کاملIntroduction to super-resolution microscopy.
In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution. The imaging property of each super-resolution technique is also compared with the correspond...
متن کاملSuper - resolved fluorescence microscopy
THE ROYAL SWEDISH ACADEMY OF SCIENCES has as its aim to promote the sciences and strengthen their influence in society.
متن کاملReview of super-resolution fluorescence microscopy for biology.
Several methodologies have been developed over the past several years for super-resolution fluorescence microscopy including saturated structured-illumination microscopy (SSIM), stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), fluorescence photoactivation localization microscopy (FPALM), and stochastic optical reconstruction microscopy (STORM). Whi...
متن کاملMotion Analysis of Live Objects by Super-Resolution Fluorescence Microscopy
Motion analysis plays an important role in studing activities or behaviors of live objects in medicine, biotechnology, chemistry, physics, spectroscopy, nanotechnology, enzymology, and biological engineering. This paper briefly reviews the developments in this area mostly in the recent three years, especially for cellular analysis in fluorescence microscopy. The topic has received much attentio...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2010
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.201002018