A Genetically Distinct Thymidine Kinase in Mammalian Mitochondria
نویسندگان
چکیده
منابع مشابه
A genetically distinct thymidine kinase in mammalian mitochondria. Exclusive labeling of mitochondrial deoxyribonucleic acid.
A thymidine kinase (EC 2.7.1.21) associated with mitochondria has been demonstrated in tissue culture both in wild type mouse cells and in cells which lack the cytoplasmic thymidine kinase. The mitochondrial thymidine kinase represents approximately 1% of the total cell thymidine kinase activity in wild type cells grown in culture and all of the activity of the mutant cells. The mitochondrial e...
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The cell line LM(TK(-)) Cl 1D, a derivative of mouse L fibroblasts deficient in thymidine kinase (EC 2.7.1.21) that shows very little thymidine kinase activity in extracts of whole cells as compared to the parental line, and that does not incorporate thymidine or 5-bromodeoxyuridine into nuclear DNA, has maintained the capacity to incorporate these precursors into mitochondrial DNA at a substan...
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Background: Most of the amino acids are encoded by more than one codon, termed as synonymous codons. Synonymous codon usage is not random as it is unique to species. In each amino acid family, some synonymous codons are preferred and this is referred to as synonymous codon usage bias (SCUB). Trends associated with evolution of SCUB and factors influencing its diversification in plastomes of gen...
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متن کاملHuman Thymidine Kinase
Cytosolic thymidine kinase (EC 2.7.1.21) has been purified 5200-fold to apparent homogeneity from normal human placenta. The purification includes sequential affinity chromatography on blue-Sepharose and a thymidine column. The molecular weight of the enzyme determined by gel filtration and sucrose density ultracentrifugation is 92,000. The subunit molecular weight is 44,000, suggesting that t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44066-0