A General Chemical Ligation Approach Towards Isopeptide-Linked Ubiquitin and Ubiquitin-Like Assay Reagents
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چکیده
منابع مشابه
A General Chemical Ligation Approach Towards Isopeptide-Linked Ubiquitin and Ubiquitin-Like Assay Reagents
Ubiquitin (Ub) and ubiquitin-like proteins (Ubls) form a family of small and highly conserved post-translational modifiers that become linked to target proteins and thus modulate their function (such as degradation, trafficking and signalling). The linkage between a Ub(l) and a target protein most frequently consists of an isopeptide bond between the C-terminal carboxylate of Ub(l) and the e-am...
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Modification of proteins with ubiquitin (Ub) occurs through a variety of topologically distinct Ub linkages, including Ube2W-mediated monoubiquitylation of N-terminal alpha amines to generate peptide-linked linear mono-Ub fusions. Protein ubiquitylation can be reversed by the action of deubiquitylating enzymes (DUBs), many of which show striking preference for particular Ub linkage types. Here,...
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2011
ISSN: 1439-4227
DOI: 10.1002/cbic.201100706