A Further Studies on Purified Hepatitis Virus
نویسندگان
چکیده
منابع مشابه
Further studies on partially purified calf thymus DNA polymerase a.
Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the...
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Different samples of purified tobacco mosaic virus show a relatively wide variation in solubility in ammonium sulfate solution. This variation and the type of solubility curve obtained in the presence of varying amounts of solid phase show that the purified virus whether isolated by mild treatment with ammonium sulfate or by ultracentrifugation is not a homogeneous chemical substance but contai...
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HBs vaccines were prepared by three different methods: 1) Heat inactivation (Krugman, et.al 1971)2) Ammonium sulphate precipitation followed by column chromatography (Sephadex G-200) 3) PEG-6000 (polyethylene glycol) precipitation followed by column chromatography using Sephadex G-200. Their efficacy was studied in guinea pigs, and the results compared with commercial Japanese vaccine (Gr...
متن کاملIsolation of single stranded DNA from purified hepatitis A-virus.
Hepatitis A-virus was purified from human stools by three purification steps. Virus was identified by radioimmunoassay and purity monitored with immune electron microscopy. Virus particles, serologically and morphologically identical, banded in CsCl in two density ranges at 1.31-1.34 g/cm3 and at 1.41-1.43 g/cm3. Virions of density 1.31-1.34 g/cm3 were shown to contain single-stranded DNA of di...
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Introduction Purification of mouse hepatitis virus (MHV) has not been succeeded until recently. It has been stated that mice infected with MHV produced the interferon-like substances within the host cells, and it produced remarkable disturbance of typical infectious cycles and symptoms (ALTUCCI 1959). FANTON1 (1964) reported that he could be able to purify the MHV ,partially by the use of fluor...
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ژورنال
عنوان ژورنال: Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
سال: 1959
ISSN: 0030-1558,1882-4528
DOI: 10.4044/joma1947.71.9-2_5791