A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
نویسندگان
چکیده
منابع مشابه
Microwave sterilization of plastic tissue culture vessels for reuse.
A simple protocol has been developed for recycling plastic tissue culture vessels. The killing properties of microwaves were used to decontaminate plastic tissue culture vessels for reuse. Nine bacterial cultures, four gram-negative and five gram-positive genera, including two Bacillus species, were used to artificially contaminate tissue culture vessels. The microwaves produced by a "home-type...
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For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of shoot structures are all cytokinin-dependent processes. Habituation refers to a n...
متن کاملHistory of plant tissue culture.
Plant tissue culture, or the aseptic culture of cells, tissues, organs, and their components under defined physical and chemical conditions in vitro, is an important tool in both basic and applied studies as well as in commercial application. It owes its origin to the ideas of the German scientist, Haberlandt, at the beginning of the twentieth century. The early studies led to root cultures, em...
متن کاملHistory of Plant Tissue Culture
CONTENTS History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identif...
متن کاملElicitors in Plant Tissue Culture
Plants or plant cells in vitro, show physiological and morphological response to microbial, physical or chemical factors which are known as ‘elicitors’. Elicitation is a process of induced or enhanced synthesis of secondary metabolites by the plants to ensure their survival persistence and competitiveness. The application of elicitors, which is currently the focus of research, has been consider...
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ژورنال
عنوان ژورنال: International Journal of Environmental Research and Public Health
سال: 2021
ISSN: 1660-4601
DOI: 10.3390/ijerph18052282