A Fluorogenic Covalent Chromone-Based Intercalator with a Mega-Stokes Shift for Sensing DNA Hybridization
نویسندگان
چکیده
Forced intercalation (FIT) probes have proven to be a reliable, rapid, inexpensive, and accurate method for the detection visualization of specific nucleic acid sequences. The accommodation rationally designed chromone-based fluorogen within double-stranded DNA structure was investigated by UV–Vis spectrophotometry steady-state fluorescence spectroscopy under physiological pH conditions. After selective excitation matching with 350 nm laser, intrinsically negligible tethered electroneutral label in single-stranded context increased 10-fold upon duplex formation. This enhancement also accompanied mega-Stokes shift (~100 nm) that placed emission cyan color range; both features are appreciable bio-imaging purposes. In sum, its fluorogenic behavior marginal impact on double helix make this dye prospective tool selectively sensing sequences interest remarkable ON/OFF contrast.
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2023
ISSN: ['2227-9040']
DOI: https://doi.org/10.3390/chemosensors11030161