A fluorescence-based assay for N-myristoyltransferase activity
نویسندگان
چکیده
منابع مشابه
Direct fluorescence-based lipase activity assay.
Lipases and esterases (glycerol ester hydrolases, E.C. 3.1.1.-) are enzymes acting on the carboxyl ester bonds present in acylglycerols. They have been recognized as useful biocatalysts because of their wide-ranging versatility in industrial applications (2,5). However, the physical properties of lipids have caused a great difficulty in studying lipolytic enzymes (4). Traditionally, lipases hav...
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Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N OW8, Canada (PS, SK, RKS); Cancer Research Unit, Saskatchewan Cancer Agency, 20 Campus Drive, Saskatoon, SK S7N 4H4, Canada (PS, SK, RKS); Drug Design and Discovery Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C9, Cana...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2012
ISSN: 0003-2697
DOI: 10.1016/j.ab.2011.10.013