A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair

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CRISPR-Cas system provides the adaptive immunity against invading genetic elements in prokaryotes. Recently, we demonstrated that Csa3a regulator mediates spacer acquisition in Sulfolobus islandicus by activating the expression of Type I-A adaptation cas genes. However, links between the activation of spacer adaptation and CRISPR transcription/processing, and the requirement for DNA repair gene...

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Clustered regularly interspaced short palindromic repeats (CRISPR) loci are arrays of short repeats separated by equally short ‘‘spacer’’ sequences [1–3]. Along with the CRISPRassociated (cas) genes, they encode an adaptive immune system of archaea and bacteria that protects the cell against viral infection [4]. Remarkably, this system is capable of inserting a short piece of an infecting viral...

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Approximately all sequenced archaeal and half of eubacterial genomes have some sort of adaptive immune system, which enables them to target and cleave invading foreign genetic elements by an RNAi-like pathway. CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems consist of the CRISPR loci with multiple copies of a short repeat sequence separa...

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ژورنال

عنوان ژورنال: Molecular Microbiology

سال: 2010

ISSN: 0950-382X

DOI: 10.1111/j.1365-2958.2010.07465.x