A DNA replication mystery

DNA REPLICATION AND SYNTHESIS OF DNABINDING PROTEINS IN THE CHLOROPLASTS OF A CALLUS CULTURE

Continuous labelling of callus with H-thymidine results in intermittent peaks of H-DNA per chloroplast, showing synchrony of division. The increase in H-DNA could be due to several replication rounds, and the drop to successive plastid divisions without intervening DNA synthesis. The level of DNA-binding proteins in the chloroplast parallels the peaks of plastidal DNA synthesis; such pro...

Peeling back the mystery of DNA overstretching.

W hen a single DNA molecule is stretched beyond its normal B-form contour length, it will undergo one of two types of conformational transitions at high or low force, depending on its attachment and ability to rotate. The structure of the DNA during these transitions is difficult to measure with traditional structural methods because it only occurs on a single molecule. More than a decade after...

Replication of a DNA microarray.

A mechanical method for efficient replication of DNA microarrays is described. The approach consists of three steps. First, a master DNA microarray consisting of single-stranded DNA elements is exposed to a solution containing the biotin-functionalized complement of each array element. Following hybridization, a replica surface modified with streptavidin is brought into contact with the master....

DNA replication.

DNA replication: a complex matter.

In eukaryotic cells, the essential function of DNA replication is carried out by a network of enzymes and proteins, which work together to rapidly and accurately duplicate the genetic information of the cell. Many of the components of this DNA replication apparatus associate with other cellular factors as components of multiprotein complexes, which act cooperatively in networks to regulate cell...