A deoxyribozyme that synthesizes 2',5'-branched RNA with any branch-site nucleotide
نویسندگان
چکیده
منابع مشابه
A deoxyribozyme that synthesizes 20,50-branched RNA with any branch-site nucleotide
RNA molecules with internal 20,50-branches are intermediates in RNA splicing, and branched RNAs have recently been proposed as retrotransposition intermediates. A broadly applicable in vitro synthetic route to branched RNA that does not require selfsplicing introns or spliceosomes would substantially improve our ability to study biochemical processes that involve branched RNA. We recently descr...
متن کاملA deoxyribozyme that synthesizes 2′,5′-branched RNA with any branch-site nucleotide
RNA molecules with internal 2',5'-branches are intermediates in RNA splicing, and branched RNAs have recently been proposed as retrotransposition intermediates. A broadly applicable in vitro synthetic route to branched RNA that does not require self-splicing introns or spliceosomes would substantially improve our ability to study biochemical processes that involve branched RNA. We recently desc...
متن کاملA deoxyribozyme that forms a three-helix-junction complex with its RNA substrates and has general RNA branch-forming activity.
We recently used in vitro selection to identify 7S11, a deoxyribozyme that synthesizes 2',5'-branched RNA. The 7S11 DNA enzyme mediates the nucleophilic attack of an adenosine 2'-hydroxyl group at a 5'-triphosphate, forming 2',5'-branched RNA in a reaction that resembles the first step of in vivo RNA splicing. Here, we describe 7S11 characterization experiments that have two important implicati...
متن کاملAdenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing.
We previously used in vitro selection to identify the 7S11 deoxyribozyme, which catalyzes formation of 2',5'-branched RNA using a branch-site adenosine nucleophile and a 5'-triphosphate electrophile. An unanswered question is whether the use of branch-site adenosine is inherently preferred or a chance event during the particular selection experiment. Here we have found that deoxyribozymes newly...
متن کاملOptimization and generality of a small deoxyribozyme that ligates RNA.
In vitro evolution was previously used to identify a small deoxyribozyme, 7Q10, that ligates RNA with formation of a 2'-5' phosphodiester linkage from a 2',3'-cyclic phosphate and a 5'-hydroxyl group. Ligation occurs in a convenient "binding arms" format analogous to that of the well-known 10-23 and 8-17 RNA-cleaving deoxyribozymes. Here, we report the optimization and generality of 7Q10 as a 2...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gki656