A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
نویسندگان
چکیده
منابع مشابه
MicroRNA Modulation during the In vitro Culture of Hematopoietic Stem Cells Prior to Transplantation
Background: Human umbilical cord blood (HUCB) is an acceptable and readily accessible source of stem cells. There is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated HUCB cells. The involvement of miRNAs in several biological processes has been shown. The aim of this study was to ev...
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Human CD34+/Thy-1+/Lin- hematopoietic cells purified from bone marrow (BM) or mobilized peripheral blood (MPB) are highly enriched for pluripotent stem cells. Ex vivo expansion of this population is proposed as a means of providing accelerated short-term, as well as long-term, engraftment after myeloablative therapy. Here we demonstrate that primitive quiescent cells are retained in bulk expans...
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متن کاملCulture of Hematopoietic Stem and Progenitor Cells
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2021
ISSN: 1940-087X
DOI: 10.3791/61938