A conceptual synthetic membrane system for active transport.

Conceptual developments in membrane transport, 1924-1974.

Optimizing Diffusive Transport Through a Synthetic Membrane Channel

Position x P1 P2 P3 Transport of ions, [ 1 , 2 ] proteins, [ 3 ] antibiotics [ 4 , 5 ] and other macromolecular solutes through channels and pores is ubiquitous in nature. In particular channel-facilitated diffusion relies on optimized binding sites for the transported particles inside the channel. [ 6 ] Well characterized examples include membrane channels such as maltoporins [ 7 , 8 ] or aqua...

Characterization of an Active Transport System for Calcium in Inverted Membrane Vesicles of Escher&k coZi*

The energy-dependent uptake of calcium by inverted membrane vesicles of Escherichia coli was investigated. Methods for preparation and storage of the vesicles were devised to allow for the maximal activity and stability of the calcium transport system. The pH and temperature optima for the reaction were observed to occur at pH 8.0 and 30”, respectively. The effects of various ions on the transp...

Characterization of an active transport system for calcium in inverted membrane vesicles of Escherichia coli.

The energy-dependent uptake of calcium by inverted membrane vesicles of Escherichia coli was investigated. Methods for preparation and storage of the vesicles were devised to allow for the maximal activity and stability of the calcium transport system. The pH and temperature optima for the reaction were observed to occur at pH 8.0 AND 30 DEGREES, RESPECTIVELY. The eft was found that the extent ...

A derepressible active transport system for glucose in Neurospora crassa.

Two glucose uptake systems have been detected in ungerminated conidia of wild type Neurospora crassa, strain 74A; the high &ty system has been characterized. Uptake and substrate competition experiments showed that the high &inity system was relatively speciiic for D-glucose but also trsnsported the nonmetabolizable analogues 6-deoxy-D&ucase, 2-deoxy-D-glucose, and 3-O-methyl-D-glucose. The sys...