A computer-driven approach to PCR-based differential screening, alternative to differential display
نویسندگان
چکیده
منابع مشابه
A computer-driven approach to PCR-based differential screening, alternative to differential display
MOTIVATION Polymerase chain reaction (PCR)-based RNA fingerprinting is a powerful tool for the isolation of differentially expressed genes in studies of neoplasia, differentiation or development. Arbitrarily primed RNA fingerprinting is capable of targeting coding regions of genes, as opposed to differential display techniques, which target 3' non-coding cDNA. In order to be of general use and ...
متن کاملDifferential Display-PCR.
This method uses PCR to amplify and display many cDNAs derived from the mRNAs of a given cell or tissue type. The method relies on two different types of synthetic oligonucleotides: anchored antisense primers and arbitrary sense primers. A typical anchored primer is complementary to approx. 13 nucleotides of the poly(A) tail of mRNA and the adjacent two nucleotides of the transcribed sequence. ...
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ICON is a computer-based expert system being developed to help radiologists with the process of differential diagnosis. ICON focuses on the domain of lung disease as seen on a chest radiograph in patients with lymphoproliferative disorders. Rather than attempt to tell the radiologist what the diagnosis is, the system asks him or her to propose a diagnosis and then discusses the evidence support...
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We previously described a targeted genomic differential display method (TGDD: Broude NE, Chandra A, Smith CL. Differential display of genomic subsets containing specific interspersed repeats. Proc. Natl. Acad. Sci. USA 1997;94:4548-53). In that method, presently characterized as method I, targeting was accomplished by capturing DNA fragments containing specific a sequence by hybridization with ...
متن کاملDifferential screening of gene expression difference enriched by differential display.
Since its original description in 1992 (1), differential display has become a widely used methodology for cloning differentially expressed genes. Much effort has since been made to further optimize and streamline the technique (2,3). However, one major bottle neck remaining for the method lies at the screening step for the verification of the cDNA fragments identified by differential display. T...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 1999
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/15.2.93