A Comparison of Penicillin-producing Strains of Penicillium notatum-chrysogenum
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چکیده
منابع مشابه
A Comparison of Penicillin-producing Strains of Penicillium notatum-chrysogenum.
Sampling and storage. The fermentations were conducted in the 100-gallon tanks described by Stefaniak et al. (1946). Samples were removed periodically into 500-ml flasks and ifitered. A 0.5-ml filtered sample was immediately diluted for penicillin assay by addition to previously chilled phosphate buffer in volumetric flasks (4.0 g KH2P04 and 1.0 g K2HPO4 per 500 ml solution in distilled water; ...
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A great deal of work has been directed toward increasing the yield of penicillin in fermentations with Penicillium chrysogenum. The spectacular increase in yield which has been achieved has resulted, in the main, from two approaches. In the first approach, mutant strains of P. chrysogenum produced by spontaneous variation or by the action of mutagenic agents such as nitrogen mustard or ultravio...
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The development and study of strains of Penicillium chrysogenum that give high yields of penicillin has been a major project at the University of Wisconsin during the past 12 years. Many thousands of cultures have been screened for their penicillin-producing ability in the Botany Department of the University. The methods used for the production and screening of mutants, the genealogy of the out...
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Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...
متن کاملFrom Two Strains of Penicillium Notatum
This paper is the second of a series dealing with the analysis of penicillin by means of a chromatogram using solvent-phosphate buffer partition on a silica base. The first paper (Levi, 1948) described the development of this type of chromatogram which is based on that ofMartin & Synge (1941), and outlined the principles of its application to the study of penicillin. The work described was carr...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1946
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.52.1.129-140.1946