A Combinatorial Polymer Library Approach Yields Insight into Nonviral Gene Delivery
نویسندگان
چکیده
منابع مشابه
Topic Introduction Nonviral Gene Delivery
Modern cell biology increasingly relies on molecular tools to facilitate the study of cellular processes. With the advent of recombinant DNA technology, reverse transcription–polymerase chain reaction (RT-PCR) to amplify almost any gene, and fluorescent proteins that can be fused to any desired target protein, the number and scope of functional studies designed to determine the roles of protein...
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Diseases of the liver have a large impact on human health. Genetic disorders, metabolic disorders, alcoholism, cancer, or infections can all impair liver function. If serious enough, a liver transplant may be necessary, a major surgical procedure which requires life-long immune suppression and relies on the availability of donor livers. Gene therapy is being intensively studied as a potential m...
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To date, both viral and nonviral vectors have been exploited for delivery of gene-based therapies to target cells/tissues. Despite high efficiency of the viral vectors (e.g., retroviruses and adenoviruses), these vectors appear to be immunogenic and potentially harmful when used in clinical gene therapy protocols (Ferber, 2001b). Besides, the preparation and purification of the viral vectors ap...
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Optimal gene therapy for cancer must (i) deliver DNA to tumor cells with high efficiency, (ii) induce minimal toxicity, and (iii) avoid gene expression in healthy tissues. To this end, we generated a library of >500 degradable, poly(beta-amino esters) for potential use as nonviral DNA vectors. Using high-throughput methods, we screened this library in vitro for transfection efficiency and cytot...
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Natural biopolymers are widely used in the field of drug and gene delivery. In this study, alginate nanoparticles were prepared using water-in-oil reverse microemulsion as a template followed by calcium crosslinking of guluronic acid units of alginate polymer. After collected by ultracentrifugation, alginate nanoparticles were analyzed by electron microscopy to obtain the size and morphology wh...
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ژورنال
عنوان ژورنال: Accounts of Chemical Research
سال: 2008
ISSN: 0001-4842,1520-4898
DOI: 10.1021/ar7002336