A Cloned Tyrosinase Turns E. coli Black

نویسندگان

چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Black Hole Horizon and Turns it Singular

We present a static solution for d = 2 critical string theory including the tachyon T but not its potential V (T ). This solution thus incorporates tachyon back reaction and, when T = 0, reduces to the black hole solution. When T 6= 0 one finds that (1) the Schwarzschild horizon of the above black hole splits into two, resembling Reissner-Nordstrom horizons and (2) the curvature scalar develops...

متن کامل

Construction of a specialized-ribosome vector or cloned-gene expression in E. coli.

An expression system utilizing specialized ribosomes has been constructed with beta-galactosidase as the product. Ribosomes specific for lacZ mRNA are generated due to a mutation within the anti-Shine-Dalgarno region of a plasmidborne 16S rRNA gene that is complementary to a mutation within the ribosome-binding site of lacZ. Hence, a subpopulation of ribsomes specific for translation of the clo...

متن کامل

A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12.

Attaching and effacing (AE) bacteria are a diverse group of gastrointestinal pathogens, comprising members of four genera, that cause the intestinal epithelial microvilli to be replaced with raised clusters of filamentous actin that conform to the surface of attached bacteria. We have cloned a 35.4 kb 'pathogenicity island' from the prototype AE bacterium, enteropathogenic Escherichia coli, con...

متن کامل

Genetic and Eukaryotic Physical Selection of Gfnes Cloned in E. Coli

Techniques have been recently developed for the construction and cloning of viable molecular hybrids between modified bacteriophage ,t DNAs and any foreign DNA 11,2,3,4]. As shown in fig. l, one of these modifications of ,t DNA involves the genetic removal of two ,EcoRI restriction sites (sites 4 and 5), a genetic deletion of non-essential DNA in the right arm, and the biochemical deletion of t...

متن کامل

Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli.

The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and overexpressed it in E. coli. Upon induction with IPTG, M.MspI constitutes more than 10% of cellular protein. A scheme has been devised to purify large ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature Biotechnology

سال: 1990

ISSN: 1087-0156,1546-1696

DOI: 10.1038/nbt0790-603