A 3D-Printed Electrochemical Immunosensor Employing Cd/Se ZnS QDs as Labels for the Rapid and Ultrasensitive Detection of Salmonella typhimurium in Poultry Samples

Salmonella is one of the leading causes foodborne illnesses worldwide, with poultry products being a major source contamination. Thus, detection salmonella in commercial crucial to minimize effects on public health. Electrochemical sensors are promising tools for bacteria due their sensitivity, simplicity, and potential on-site analysis. In this work, three-dimensional (3D) printed electrochemical immunosensor determination typhimurium fresh chicken through sandwich immunoassay employing biotinylated anti-S. antibody followed by streptavidin labeled Cd/Se ZnS quantum dots (QDs) presented. The device features three carbon-black polylactic acid electrodes holder, quantification S. performed anodic stripping voltametric (ASV) Cd(II) released after acidic dissolution QDs. To enhance an electroplated bismuth film was deposited working electrode, achieving limit 5 cfu/mL total assay time 25 min, whereas h sample pre-enrichment required 1 cfu/25 mL rinse broth. method accurate, %recovery values ranging from 93.3 113% samples, repeatable intra- inter- coefficient variations <2 5%, respectively, indicating suitability proposed at point-of-need.