958. Sleeping Beauty-Mediated Gene Therapy for Colorectal Cancer
نویسندگان
چکیده
منابع مشابه
Sleeping beauty transposon-mediated gene therapy for prolonged expression.
The Sleeping Beauty (SB) transposon system represents a new vector for non-viral gene transfer that melds advantages of viruses and other forms of naked DNA transfer. The transposon itself is comprised of two inverted terminal repeats of about 340 base pairs each. The SB system directs precise transfer of specific constructs from a donor plasmid into a mammalian chromosome. The excision of the ...
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The aim of this study was to use gene therapy via the Sleeping Beauty (SB) system to increase telomerase promoter activity to target hepatocellular carcinoma (HCC). In previous studies, we identified selective and increased expression of luciferase and suicide genes controlled by the hTERT (human telomerase reverse transcriptase) promoter and the SV40 enhancer in telomerase-positive cancer cell...
متن کاملEffective Gene Trapping Mediated by Sleeping Beauty Transposon
Gene trapping is a high-throughput approach to elucidate gene functions by disrupting and recapitulating expression of genes in a target genome. A number of transposon-based gene-trapping systems are developed for mutagenesis in cells and model organisms, but there is still much room for the improvement of their efficiency in gene disruption and mutation. Herein, a gene-trapping system mediated...
متن کاملThe Sleeping Beauty transposon system: a non-viral vector for gene therapy.
Over the past decade, the Sleeping Beauty (SB) transposon system has been developed as the leading non-viral vector for gene therapy. This vector combines the advantages of viruses and naked DNA. Here we review progress over the last 2 years in vector design, methods of delivery and safety that have supported its use in the clinic. Currently, the SB vector has been validated for ex vivo gene de...
متن کاملHorgan on Sleeping Beauty
With the notable exception of David Lewis, most of those writing on the Sleeping Beauty problem have argued that 1/3 is the correct answer. Terence Horgan has provided the clearest account of why, contrary to Lewis, Beauty has evidence against the proposition that the coin comes up heads when she awakens on Monday. In this paper, I argue that Horgan’s proposal fails because it neglects importan...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2006
ISSN: 1525-0016
DOI: 10.1016/j.ymthe.2006.08.1050