616 Ultraviolet A mediates the keratinocytes supranuclear melanin cap formation via opsin 3

Background: the human keratinocytes supranuclear melanin cap acts as a microparasol to protect nucleus from ultraviolet (UV) induced DNA damage. UV cytoplasmic dynein expression mediates formation. However, molecular mechanism of responding irradiation and mediating formation remains unclear. Opsins(OPNs) belong G protein-coupled receptors superfamily are photosensitive receptor proteins phototransduction by translation absorbed photons cellular responses. OPN3 is member opsin family widely expressed in mammalian tissues. Its absorption spectrum function remain Objective: To investigate whether can mediate UVA induce Methods: We detected primary keratinocyte HaCaT real-time fluorescence quantitative PCR Western blot, observed localization confocal microscopy. silencing with small interfering RNA technology lentivirus transfection technology, After, was Fontana-Masson silver method protocol. And related signal pathway studied blot. Results: important light sensor responsible for mediated OPN3-mediated required Ca2+-dependent Gai cyclic adenosine monophosphate (CAMP) transduction, thus contributing UVA-induced AKT phosphorylation upregulated expression, providing evidence phototransduction. Conclusion: our study provides insights into mechanisms which respond radiation Formation may further reveal physiological role skin response light.