3P031 Protein Stability Analysis of MHC/peptide Complex from Xray Single Molecule Tracking(01A. Protein: Structure,Poster)
نویسندگان
چکیده
منابع مشابه
Nanofluidic Networks for Single Molecule Protein Analysis
Overview A robust, low cost, and reproducible process for polymer nanochannel fabrication based on thermomechanical deformation is being developed, with application to nanofluidic biomolecular separations followed by single molecule detection. A critical issue in singlemolecule analysis is the ability to spatially constrain molecules of interest within a nanoscale confinement zone. Spatial loca...
متن کاملCorrelation Analysis of Enzymatic Reaction of a Single Protein Molecule.
New advances in nano sciences open the door for scientists to study biological processes on a microscopic molecule-by-molecule basis. Recent single-molecule biophysical experiments on enzyme systems, in particular, reveal that enzyme molecules behave fundamentally differently from what classical model predicts. A stochastic network model was previously proposed to explain the experimental disco...
متن کاملQuantitative analysis of single-molecule RNA-protein interaction.
RNA-binding proteins impact gene expression at the posttranscriptional level by interacting with cognate cis elements within the transcripts. Here, we apply dynamic single-molecule force spectroscopy to study the interaction of the Arabidopsis glycine-rich RNA-binding protein AtGRP8 with its RNA target. A dwell-time-dependent analysis of the single-molecule data in combination with competition ...
متن کاملUltrafast dynamics of protein collapse from single-molecule photon statistics.
We use the statistics of photon emission from single molecules to probe the ultrafast dynamics of an unfolded protein via Förster resonance energy transfer. Global reconfiguration of the chain occurs on a time scale of approximately equal to 50 ns and slows down concomitant with chain collapse under folding conditions. These diffusive dynamics provide a missing link between the phenomenological...
متن کاملSensitive single-molecule protein quantification and protein complex detection in a microarray format.
Single-molecule protein analysis provides sensitive protein quantitation with a digital read-out and is promising for studying biological systems and detecting biomarkers clinically. However, current single-molecule platforms rely on the quantification of one protein at a time. Conventional antibody microarrays are scalable to detect many proteins simultaneously, but they rely on less sensitive...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2013
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.53.s217_1