3D Printed Scaffolds Incorporated with Platelet‐Rich Plasma Show Enhanced Angiogenic Potential while not Inducing Fibrosis

Successful therapeutic strategies for wound healing rely on proper vascularization while inhibiting fibrosis. However, scaffolds designed skin tissue engineering generally lack the biochemical cues that can enhance their without inducing Therefore, objective of this work is to incorporate platelet-rich plasma (PRP), a natural source angiogenic growth factors, into gelatin methacrylate (GelMA) hydrogel, yielding bioink subsequently be used 3D print novel regenerative scaffold with defined architecture healing. A PRP-activated successfully printed, and resulting present similar structural, rheological, mechanical properties compared GelMA-only scaffolds. Furthermore, printed facilitate controlled release PRP-derived factors up 14 days, presenting superior potential in vitro (e.g., tubulogenesis assay) vivo (chick chorioallantoic membrane) scaffolds, not myofibroblastic phenotype fibroblasts α-smooth muscle actin expression). This disruptive technology offers opportunity patient's autologous incorporated tailored 3D-printed theatre prior implantation, as part single-stage procedure, has other applications which enhanced limited fibrosis desired.