3D microchannel co-culture: method and biological validation
نویسندگان
چکیده
منابع مشابه
3D microchannel co-culture: method and biological validation.
Conventional 3D culture is typically performed in multi-well plates (e.g. 12 wells). The volumes and dimensions necessitate relatively large numbers of cells and fluid exchange steps are not easily automated limiting throughput. 3D microchannel culture can overcome these challenges simplifying 3D culture processes. However, the adaptation of immunocytochemical endpoint measurements and the vali...
متن کاملBag culture: a method for root-root co-culture.
A method named "bag culture" was developed for coculturing of Linum persicum (section Syllinum) and L. austriacum (section Linum) hairy roots. For this propose L. austriacum and L. persicum hairy root cultures were established using Agrobacterium rhizogenes in McCown medium. L. persicum hairy roots in bags (1 mm2 mesh) were successfully grown together with L. austriacum hairy roots. The amounts...
متن کاملFabrication of a Circular Pdms Microchannel to Construct 3d Culture Microsystem
In this study, we described the fabrication method for microfluidic channels with circular-cross sectional geometries by reflow phenomenon. The photoresist reflow leads to shape change from rectangular to semicircular, and we optimized the reflow conditions by using positive photoresist to form the perfect cylindrical micropatterns. The resultant circular micropattern was used as a template for...
متن کاملPreparation of Decellularized Biological Scaffolds for 3D Cell Culture.
The biggest challenge of designing and implementing an in vitro study is developing a microenvironment that most closely represents the interactions observed in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM ) proteins necessary ...
متن کاملSimulation of fluid slip at 3D hydrophobic microchannel walls by the lattice Boltzmann method
Fluid slip along hydrophobic microchannel walls has been observed experimentally by Tretheway and Meinhart [Phys. Fluids, 14 (3) (2002) L9]. In this paper, we show how fluid slip can be modeled by the lattice Boltzmann method and investigate a proposed mechanism for the apparent fluid slip [Phys. Fluids (2003)]. By applying an exponentially decaying hydrophobic repulsive force of 4 · 10 3 dyn/c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Integrative Biology
سال: 2010
ISSN: 1757-9694,1757-9708
DOI: 10.1039/c0ib00001a