3D engineered neural co-culture model and neurovascular effects of marine fungi-derived citreohybridonol

Marine-based biomolecules are emerging metabolites that have gained attention for developing novel biomaterials, drugs, and pharmaceutical in vitro platforms. Here, we developed a 3D engineered neural co-culture model via prototyped sliding frame-platform multi-step UV lithography investigated the neurovascular potential of citreohybridonol neuroblastoma treatment. Citreohybridonol was isolated from sponge-derived fungus Penicillium atrovenetum. The characterized by Fourier-transform infrared spectroscopy scanning electron microscopy analysis. Human umbilical cord vein endothelial cells (HUVECs) (SH-SY5Y) cell lines were encapsulated gelatin methacrylate (GelMA) with without citreohybridonol. effect on proliferation capacity assessed viability immunostaining assays. GelMA culture characterization indicated successfully as axenic mixed with/without cytotoxic test confirmed microenvironment non-toxic cultural experiments, it showed inhibitory effects SH-SY5Y induced HUVECs. Finally, immunohistochemical staining demonstrated suppressed vascularization HUVECs culture.