394. Neuron-Specific, In Vivo Gene Delivery Mediated by Non-Viral Vectors
نویسندگان
چکیده
منابع مشابه
Strategies to test nuclear localization of non-viral gene delivery vectors in vitro and in vivo
Non-viral gene delivery is plagued by low transfection levels compared to viral delivery. The nuclear envelope presents a significant obstacle for non-viral vectors. A peptide-based nuclear localizing sequence has been incorporated into non-viral vectors to traverse the nuclear envelope. Here, we selected a photo-chemical method for covalently labeling the peptide onto plasmid DNA. The hypothes...
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In principle, the technique of gene delivery involves taking complete or parts of genes that can code specific message and delivering them to selected cells in the body. Such a transfer of plasmid DNA into mammalian cells has posed major challenges for gene therapy. This study shows the encapsulation of a plasmid DNA in cross-linked polyvinylpyrrolidone (PVP) nanoparticles of size less than 100...
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INTRODUCTION:• Gene delivery into joints is considered to become a useful strategy for the treatment of joint diseases. Viral vectors are commonly used because of their high transduction efficacies. Despite of recent improvement of the vectors, the viral vectors are associated with some unsolved problems such as induction of inflammatory responses and undesired generation of replication-compete...
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Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in CFTR genes that affect chloride ion channel. The CF is a good nominee for gene therapy as the asymptomatic carriers are phenotypically normal, and the desired cells are accessible for vector delivery. Gene therapy shows promising effects involving the correction of gene or replacement of the mutant gene with the func...
متن کاملIn vivo gene delivery and expression by bacteriophage lambda vectors
AIMS Bacteriophage vectors have potential as gene transfer and vaccine delivery vectors because of their low cost, safety and physical stability. However, little is known concerning phage-mediated gene transfer in mammalian hosts. We therefore performed experiments to examine phage-mediated gene transfer in vivo. METHODS AND RESULTS Mice were inoculated with recombinant lambda phage containin...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2006
ISSN: 1525-0016
DOI: 10.1016/j.ymthe.2006.08.456