361 Assessment of Nomadic and Niche-specialist Lactobacilli as Potential Vaginal Probiotics
نویسندگان
چکیده
Recent studies have shown that the microbiota during early years of an individual’s life is affected by mode release childbirth, differing between caesarean section and newborns born vaginally. Here we reported selection nomadic lactobacilli for development a multi-strain probiotic gel to promote lactobacilli-dominated vaginal in pregnant women establish proper eubiosis new-born. Lactobacilli, mainly isolated from food sources, were screened functional characteristics capability inhibit Streptococcus agalactiae Candida albicans, which may lead adverse pregnancy-related outcomes. A total ninety-five strains (Lacticaseibacillus paracasei or Lactiplantibacillus plantarum) auto-aggregation, hydrogen peroxide production, hydrophobicity, lactic acid isomers quantification. Cell-free supernatants (CFSs) candidate also co-inoculated with pathogens high-throughput inhibition screening. The best performing vitro, alone combination, on SkinEthicTM HVE model, evaluate reduction expression genes (interleukin-8, interleukin-6 tumour-necrosis factor alpha) involved inflammatory cascade. We found production was strain-dependent, highest concentrations L. paracasei. plantarum produced both acid, while Lb. only L-isomer. Hydrophobicity auto-aggregation higher than 50% observed respectively 25% 86% all strains. S. strongly inhibited wide range CFSs, whereas C. albicans less frequent. Two showed Overall, had pathogen score traits.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2022
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2022.09.374