3′ Nontranslated RNA Signals Required for Replication of Hepatitis C Virus RNA

3' nontranslated RNA signals required for replication of hepatitis C virus RNA.

We describe a mutational analysis of the 3' nontranslated RNA (3'NTR) signals required for replication of subgenomic hepatitis C virus (HCV) RNAs. A series of deletion mutants was constructed within the background of an HCV-N replicon that induces the expression of secreted alkaline phosphatase in order to examine the requirements for each of the three domains comprising the 3'NTR, namely, the ...

Nontranslated RNA Signals Required for Replication of Hepatitis C Virus RNA

We describe a mutational analysis of the 3 nontranslated RNA (3 NTR) signals required for replication of subgenomic hepatitis C virus (HCV) RNAs. A series of deletion mutants was constructed within the background of an HCV-N replicon that induces the expression of secreted alkaline phosphatase in order to examine the requirements for each of the three domains comprising the 3 NTR, namely, the h...

Sequences in the 5' nontranslated region of hepatitis C virus required for RNA replication.

Sequences in the 5' and 3' termini of plus-strand RNA viruses harbor cis-acting elements important for efficient translation and replication. In case of the hepatitis C virus (HCV), a plus-strand RNA virus of the family Flaviviridae, a 341-nucleotide-long nontranslated region (NTR) is located at the 5' end of the genome. This sequence contains an internal ribosome entry site (IRES) that is loca...

Virol . Bartenschlager Peter Friebe , Volker Lohmann , Nicole Krieger and Ralf Hepatitis C Virus Required for RNA Replication Nontranslated Region of ′

Genetic analysis of sequences in the 3' nontranslated region of hepatitis C virus that are important for RNA replication.

The genome of the hepatitis C virus (HCV) is a plus-strand RNA molecule that carries a single long open reading frame. It is flanked at either end by highly conserved nontranslated regions (NTRs) that mediate crucial steps in the viral life cycle. The 3' NTR of HCV has a tripartite structure composed of an about 40-nucleotide variable region, a poly(U/UC) tract that has a heterogeneous length, ...