3-Methyleneoxindole Reductase of Peas
نویسندگان
چکیده
منابع مشابه
3-Methyleneoxindole reductase of peas.
A 100-fold purification of a reduced triphosphopyridine nucleotide/3-methyleneoxindole reductase of peas has been achieved using conventional protein fractionation procedures. Reduced diphosphopyridine nucleotide is 25-fold less effective than reduced triphosphopyridine nucleotide as the reductant. The preparation is free of other reductase activities including those linking the oxidation of re...
متن کاملInhibition of cellular and viral protein synthesis by 3-methyleneoxindole.
The effect of 3-methyleneoxindole (MO) on mengovirus and L-cell protein synthesis was investigated. MO was found to inhibit mengovirus multiplication and the incorporation of radioactive amino acids into both viral and cellular proteins. These results suggest that the antiviral effect of this compound is not specific but rather stems from its inhibition of the cellular translational machinery u...
متن کاملInhibitory oxidation products of indole-3-acetic Acid: 3-hydroxymethyloxindole and 3-methyleneoxindole as plant metabolites.
Extracts of pea seedlings (Pisum sativum, variety Alaska) oxidize indole-3-acetic acid to a bacteriostatic compound which has been identified as 3-hydroxymethyloxindole. At physiological pH this compound is readily dehydrated to 3-methyleneoxindole, another bacteriostatic agent. The extracts of pea seedlings also contain a reduced triphosphopyridine nucleotide-linked enzyme which reduces 3-meth...
متن کاملThe Binding of Indole-3-acetic Acid and 3-Methyleneoxindole to Plant Macromolecules.
Homogenates of pea (Pisum sativum L., var. Alaska) seedlings exposed to (14)C-indole-3-acetic acid or (14)C-3-methyleneoxindole, an oxidation product of indole-3-acetic acid, were extracted with phenol. In both cases 90% of the bound radioactivity was found associated with the protein fraction and 10% with the water-soluble, ethanol-insoluble fraction. The binding of radioactivity from (14)C-in...
متن کاملInactivity of 3-methyleneoxindole as mediator of auxin action on cell elongation.
The recently reported growth-promoting ability of 3-methyl-eneoxindole was examined in order to test the hypothesis that indole-3-acetic acid acts as a growth promoter only after oxidative conversion to 3-methyleneoxindole. Methyleneoxindole was synthesized from indole-3-acetic acid and N-bromosuccinimide, and its identity was confirmed by ultraviolet absorption, infrared absorption, mass spect...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1967
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.42.4.510