27P MiR-939-5p exhibits tumour suppressor activity and immune surveillance manipulation in triple-negative breast cancer

MiRNAs have been thoroughly studied for their vast roles in oncology, however, recent immune-related miRNAs recognized as prospective modulators the tumor immune surveillance process. The high immunogenicity of TNBC along with its stubbornness towards effective targeted therapy shifted therapeutic interests immunotherapy. Accordingly, a promising approach would be targeting suppressive microenvironment (TME). Such an could manifested dual manner through blocking prominent checkpoint proteins and regulating Natural Killer (NK) cells thus modifying TME. MiR-939-5p is scarcely explored miRNA especially TNBC. Therefore, our aim to identify expression profile miR-939-5p BC tissues role it plays suppression well immunomodulatory on NKG2D ligands Breast biopsies were collected from 40 patients. MDA-MB-231 cultured transfected different oligonucleotides. Total RNA was extracted quantified by qRT-PCR. Cellular viability, colony forming ability migration measured using MTT, scratch assays, respectively. down-regulated compared normal other subtypes. Functionally, forced ensued decline cellular capacity highlighting Immunomodulation wise, overexpression resulted down-regulation protein PD-L1 upregulation shedded MICA/B cells. Lastly, drastic repression inhibitory cytokines, TNF-? IL-10 detected upon upregulation. present study categorized suppressing under expressed patients cell lines. Furthermore, institutes first major stride unraveling advancing NK cytotoxicity trimming TME favor eradication, proposing novel module