2,4-Dichlorophenoxyacetic Acid Loaded Polymeric Nanoparticle Synthesis and Its Effect on Biomass in Medicago sativa Cell Suspension Cultures

Nanoparticular systems, which have made great advances with the development of nanotechnology, been used extensively recently in pesticide, herbicide plant growth regulators, fertilizer applications, gene transfer technologies and agriculture. It was aimed to synthesize characterize 2,4-D loaded PLGA nanoparticles investigate their biological activity comparison its free form. Here, effects poly (lactic-co-glycolic) acid (PLGA) on biomass Medicago sativa cell suspension cultures were investigated. Single emulsion solvent evaporation method is nanoparticle synthesis. As a result characterization nanoparticles, 63.82% encapsulation efficiency, 60.73% reaction efficiency 10.51% drug loading capacities calculated. Particle size measured as 181.7 ± 3.74 nm, zeta potential -18.3 1.48 polydispersity index 0.081.
 Compared molecule, it observed that addition medium using release system increased cells yield M. cultures.