226HEAT SHOCK OF GERMINAL VESICLE-STAGE BOVINE OOCYTES REDUCES EMBRYO DEVELOPMENT
نویسندگان
چکیده
منابع مشابه
Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes.
BACKGROUND Somatic cell nuclear transfer (SCNT) involves the transfer of somatic cell nuclei into enucleated oocytes. Because human in vivo matured oocytes are scarcely available, we investigated whether in vitro matured (IVM) germinal vesicle (GV) oocytes could also support preimplantation development of human cloned embryos. METHODS Three groups were used for SCNT: in vitro matured GV oocyt...
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Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse g...
متن کاملthe effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...
متن کاملP-72: Ovine Oocytes Vitrified at Germinal Vesicle Stage as Cytoplast Recipients forSomatic Cell Nuclear Transfer (SCNT)
Background: The cryopreservation of immature oocytes at the germinal vesicle (GV) stage would create an easily accessible, nonseasonal source of female gametes for research and reproduction. The present study investigated the ability of ovine oocytes vitrified at the GV stage using a cryoloop to be subsequently matured, fertilized and cultured in vitro to blastocyst-stage embryos. Materials and...
متن کاملDevelopmental competence of gametes reconstructed by germinal vesicle transplantation from fresh and cryopreserved bovine oocytes.
OBJECTIVE To evaluate the use of fresh or frozen bovine oocytes as an animal model for reconstructing artificial gametes by germinal vesicle transplantation (GVT), to study nucleocytoplasmic interaction and define clinical procedures for ooplasm donation in humans. DESIGN Prospective experimental study. SETTING University-based experimental laboratory. ANIMAL(S) Bovine oocytes from slaugh...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2004
ISSN: 1031-3613
DOI: 10.1071/rdv16n1ab226