2-Deoxy-d-galactose, a substrate for the galactose-transport system of Escherichia coli
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چکیده
منابع مشابه
Source of energy for the Escherichia coli galactose transport systems induced by galactose.
The beta-methyl-galactoside- and galactose-specific transport systems of Escherichia coli were shown by experiments involving inhibitors and the use of an adenosine triphosphatase mutant strain to utilize adenosine 5'-triphosphate or a related compound to drive active transport. These systems were shown to be unable to use the activated-membrane state. The galactose-specific transport system wa...
متن کاملHepatic galactose metabolism quantified in humans using 2-18F-fluoro-2-deoxy-D-galactose PET/CT.
UNLABELLED Accurate quantification of regional liver function is needed, and PET of specific hepatic metabolic pathways offers a unique method for this purpose. Here, we quantify hepatic galactose elimination in humans using PET and the galactose analog 2-(18)F-fluoro-2-deoxy-d-galactose ((18)F-FDGal) as the PET tracer. METHODS Eight healthy human subjects underwent (18)F-FDGal PET/CT of the ...
متن کاملTransport properties of the galactose-binding protein of Escherichia coli. Substrate-induced conformational change.
The galactose-binding protein, a necessary component of a bacterical transport system, is shown by three independent methods to undergo a conformational change upon binding of substrate. 1. A Lineweaver-Burk plot of the binding data obtained by equilibrium dialysis at a protein concentration of 0.4 mg per ml shows heterogenous behavior. Extrapolation of the data at low galactose concentrations ...
متن کاملgalactose-H+ transport protein, GaIP, of Escherichia coli
The binding of the transport inhibitor, forskolin, to the galactose-HI symporter, GalP, of Escherichia coli was evaluated by equilibrium and time-resolved fluorescence measurements. A quench in protein fluorescence of 8-12% was observed upon the binding of forskolin. The overall dissociation constant (Kd) for forskolin determined by fluorescence titration ranged between 1.2 and 2.2 suM, which i...
متن کاملThe regulation and properties of the galactose transport system in Escherichia coli K12.
This study of galactose-induced galactose transport in Escherichia coli K12 showed that in most strains two transport systems having the properties of “galactose permease” and “P-methyl galactoside permease” are responsible for the observed transport. Several strains contain only the galactose transport system but still grow normally on galactose. The regulation of this system was shown to be v...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1977
ISSN: 0264-6021
DOI: 10.1042/bj1680015