1P126 Enzymology of Q beta replicase: Template RNA catches "Q beta replicase" in a trap
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چکیده
منابع مشابه
Detection of Mycobacterium tuberculosis directly from sputum by using a prototype automated Q-beta replicase assay.
We have adapted an assay for the direct detection of Mycobacterium tuberculosis using a prototype automated instrument platform in which probes are amplified with Q-beta replicase. The assay was based on amplification of specific detector probe following four cycles of background reduction (reversible target capture) in a closed disposable pack. The assay signal was the time required for fluore...
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A sensitive, nonisotopic hybridization assay termed "dual capture" is described. The assay rapidly and specifically detects very low levels of target nucleic acids and organisms. The assay is based on the principles of sandwich hybridization, reversible target capture, and Q-Beta replicase amplification. The assay can be completed in less than 4 h, and in the described model format, it detects ...
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Amber mutant amB86 of bacteriophage Qbeta is capable of causing the production of five to eight times more viral replicase than wild-type phage. Su(-) bacteria infected with the mutant can carry the viral RNA in a plasmid-like state for many bacterial generations.
متن کاملSynthesis of complementary strands of heterologous RNAs with Qbeta replicase.
Qbeta replicase, in the presence of Mn(++), can use various RNAs as templates for synthesis of full-length RNA products and can also use Qbeta plus-strand RNA as template in the absence of host factors.
متن کاملMinimal requirements for template recognition by bacteriophage Qbeta replicase: approach to general RNA-dependent RNA synthesis.
Any oligo- or polynucleotide able to offer a C-C-C-sequence at the 3'-terminus and a second C-C-C-sequence in a defined steric position to Qbeta replicase is an efficient template. Corresponding chemical modifications convert non-template RNAs to template RNAs.
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2005
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.45.s63_2