منابع مشابه
Measurement of cytosolic calcium using 19F NMR.
Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts ar...
متن کاملMonitoring the Disassembly of Virus-like Particles by 19F-NMR
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of 19F-labeled VLPs derived from bacteriophage Qβ by 19F NMR. Analysis of resonances, under a ra...
متن کاملSimultaneous Detection of Distinct Ubiquitin Chain Topologies by 19F NMR
The dynamic interplay between ubiquitin (Ub) chain construction and destruction is critical for the regulation of many cellular pathways. To understand these processes, it would be ideal to simultaneously detect different Ub chains as they are created and destroyed in the cell. This objective cannot be achieved with existing detection strategies. Here, we report on the use of 19F Nuclear Magnet...
متن کاملHigh-resolution 19F and 1H NMR of a vinylidenefluoride telomer
The reaction products of vinylidenefluoride (VDF) with methanol as a telogen have been analysed in the solution state by H and F nuclear magnetic resonance (NMR). Highresolution F and H NMR spectra were achieved using high-power H and F decoupling respectively, giving superior resolution and revealing previously unresolved signals of the vinylidenefluoride telomer (VDFT). H and F homoand hetero...
متن کاملField-dependent 19F NMR study of sperm whale myoglobin reconstituted with a ring-fluorinated heme
A synthetic heme possessing immobilized fluorine atoms as peripheral side chains has been incorporated into the apoprotein of sperm whale myoglobin (Mb), and the field dependence of the line width of 19F NMR signals observed for the protein with S values ranging from 0 to 5/2 was analyzed in order to gain a quantitative insight into the 19F transverse relaxation mechanism in Mb with various mag...
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ژورنال
عنوان ژورنال: Journal of Physics: Conference Series
سال: 2011
ISSN: 1742-6596
DOI: 10.1088/1742-6596/324/1/012034