(188) ISOFORMS OF THE CYCLIC GMP-DEPENDENT PROTEIN KINASE ARE EXPRESSED IN HUMAN PENILE ERECTILE TISSUE

Abstract Objectives Aside from the activity of gaseous transmitter molecule nitric oxide (NO) and cyclic GMP producing enzyme soluble guanylyl cyclase (sGC) in human penile erectile tissue, intracellular receptor proteins activated by binding have been considered to play a significant role erection process. In animal models, down-regulation GMP-dependent/binding protein kinase I (cGKI) has linked on-set diabetes mellitus dysfunction (ED). Our study aimed investigate tissue (corpus cavernosum cavernous arteries) expression cGKI (isoforms alpha beta) relation smooth muscle alpha-actin, endothelial synthase (eNOS). Methods Cryostat sections were exposed primary antibodies (ABs) directed against cGKI, beta, eNOS, followed incubation with fluorochrome-labeled secondary ABs. Visualization was commenced means laser fluorescence microscopy. Western blot analysis also performed. Results Abundant immunoreactivities indicating presence beta second messenger cGMP observed trabecular musculature as well layer tiny arteries. Double staining revealed co-localization eNOS within endothelium small arterial vessels. The confirmed analysis. Conclusions Theclose isoforms key mediators NO pathway (eNOS, GMP), demonstrated conventional immunohistochemistry, provides further evidence for GMP-binding function control vascular non-vascular muscle. Conflicts Interest no conflict interest.